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		+	The log2 transformation is applied to all expression measurements in all microarrays in the currently loaded data set. The operation is applied only if all values in all microarrays are positive.  If this is not the case, an error message appears indicating that the normalization could not be performed.
	==Marker-based Centering==		==Marker-based Centering==

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Revision as of 12:40, 8 June 2010

Overview

Normalization is used to transform data in preparation for analysis. Many normalizers are oriented towards decreasing the effects of systematic differences across a set of microarrays, aiding in cross-microarray comparisons. A log transformation of the data can be used to improve its statistical distribution. geWorkbench offers a selection of pluggable normalization components (see list below). The Normalizer panel is located in the Commands/ Analysis area in the lower right side of the application.</p>

In geWorkbench, normalization alters the loaded dataset and is not reversible; the original is not retained. Normalization operations do not respect any marker or array sets that may be activated; normalization always acts on the entire data set.

The Missing Value Computation normalizer can be used to replace missing values with imputed values. Note that some analysis routines also will optionally compute replacements for missing values if needed.

Data preparation methods such as RMA and GCRMA are not directly available in geWorkbench. Affymetrix CEL files can be processed externally to geWorkbench using a program such as RMAExpress or in R/Bioconductor and then the processed data imported into geWorkbench.

A short introduction to various methods of Affymetrix data preparation is available at Affymetrix Preprocessing.

Normalizer Configuration

Some normalizers may not be loaded by default in geWorkbench. To configure which normalizers to load, use the Component Configuration Manager. It is available in the top menu-bar under Tools->Component Configuration.

Available Normalizers

geWorkbench comes with the following normalization routines installed:

Normalizer	Description
Housekeeping Genes Normalization	Normalize all values such that the averaged expression value of specified house-keeping markers is the same on each microarray.
Log2 Transformation	Applies a log2 transformation to all measurements in a microarray.
Marker-based Centering	Subtracts the mean or median measurement of a marker profile from every measurement in the profile.
Mean-variance Normalization	For every marker profile, the mean measurement of the entire profile is subtracted from each measurement in the profile and the resulting value is divided by the standard deviation.
Microarray-based Centering	Subtracts the mean or median measurement of a microarray from every measurement in that microarray.
Missing Value Computation	Replaces every missing value with either the mean value of that marker across all microarrays or with the mean measurement of all markers in the microarray where the missing value is observed.
Quantile Normalization	Adjusts expression values so that the distribution of values is the same on each microarray, though which marker has which value varies.
Threshold Normalization	All data points whose value is less than (or greater than) a user-specified minimum (maximum) value are raised (reduced) to that minimum (maximum) value.

Basic Controls

Overview

• Normalizer (menu) - select the desired normalizer.

• Save Parameters (menu) - allows selection of stored parameter settings.

• Normalize (button)- run the selected transformation.

• Save Settings - Save the current settings (see following section).

• Delete Settings- delete the currently selected parameter set.

Saving Parameters

The current parameter settings can be saved to a named parameter set. The saved set will be displayed in the pull-down menu at upper right in the component. Any number of parameter sets can be saved. Selecting a saved parameter set will load its settings. If the currently set parameters match a saved set, that set's entry will be shown in the menu.

• Save Settings - save the current settings to a new parameter set.
• Delete Settings - delete the currently selected parameter set from the menu.

Specific Controls for each Normalizer

Housekeeping Gene Normalization

The Housekeeping Gene normalization method normalizes data using the expression values of selected housekeeping genes. In particular, for each microarray, the average expression of all markers corresponding to housekeeping genes is calculated and then all expression levels within the microarray are divided by that average. To specify the housekeeping gene markers, the user can load a CSV (comma separated values) file containing these markers. The loaded markers are displayed in the Current Selected Genes list box. Markers can be moved back and forth between this list and the list at left, Excluded Genes through use of the right and left arrow buttons (< >), or simply by double-clicking on a marker in either list. The current contents of the Current Selected Genes marker set can be saved as a CSV file for later reuse.

Controls

• <, > = Shift selected markers between lists. Double-clicking on a list item will also shift it to the opposite list.
• Clear All - Clear both lists.
• Load - Load a list of markers from a comma-separated value (CSV) format file. One marker per line is also an acceptable format.
• Save - Save the list shown in the Current Selected Genes list to a file. The output file format is one marker per line.

• Right-click menu - If one right-clicks on a marker in the Current Selected Genes list, a pop-up menu appears, offering two choices:

1. Rename - Rename the marker in the list. Note that the marker name must match a marker in the currently loaded dataset.
2. Delete - Delete a marker from the list.

Missing Value Options for Housekeeping Genes

• Ignore - do not replace missing values.
• Microarray Average - missing values are replaced for the housekeeping gene normalization with the average of all values on the microarray on which the missing value was found.

Selected Genes Not Available

When you have attempted to normalize the data with genes that are not included in the data, the system generates a warning message and highlights the missing genes in yellow in the Current Selected Genes list box. An pop-up dialog box will offer the following choices:

• OK – Proceeds to normalize the data using only the markers that are present in the data set, disregarding the yellow-highlighted markers.
• Cancel – Cancels the normalization operation. The markers in the list which are not in the dataset will remain highlighted in yellow.
• Right-click menu options - right-clicking on a highlighted marker will pop-up a menu with the following choices:
  • Exclude Highlighted - Move the highlighted markers back to the Excluded Genes list on left.
  • Delete Highlighted - Delete the highlighted markers from the list.
  • Clear Highlights - Clear all highlights, leaving the list unchanged.

Log2 Transformation

The log2 transformation is applied to all expression measurements in all microarrays in the currently loaded data set. The operation is applied only if all values in all microarrays are positive. If this is not the case, an error message appears indicating that the normalization could not be performed.

Marker-based Centering

Mean-Variance Normalization

Microarray-based Centering

Missing Values Normalization

Quantile Normalization

Threshold Normalization