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Contents

Introduction

The Preferences item in the menu bar allows a number of features of geWorkbench to be configured.

MenuBar Preferences.png

When do changes take effect?

Changes to items in the preferences menu do not affect any already present primary data nodes, e.g. microarray data sets. The changes will be in force for any new data nodes loaded after a setting is changed. They will also take effect if geWorkbench is restarted.

Text Editor

The user can change the default text editor used to view data files. For example, when you right-click on a data node in the Workspace, "View in Editor" is one of the options offered.

Visualization

Heat maps, e.g. in the Color Mosaic component, can be displayed using either a relative or an absolute color scheme.

A change in this setting only takes effect the next time a new microarray data node is loaded. It does not affect the visualization of any node already loaded.

Note - Changing the relative/absolute preference will not take effect until the next time a data set is loaded.

  • Absolute
    • Red represents positive expression values, and green represents negative expression values. Black represents a value of zero. The shade represents the expression magnitude as follows:
    • Formal spec: Let M = max{|min|, |max|} over all expression measurements, across all arrays. If expression value x > 0, assign it the red spectrum x / M * 256. If expression value x is negative, assign it to the green spectrum -x / M * 256.
  • Relative (default)
    • Relative is similar to the setting for Absolute, but each marker is mean-variance normalized first. A Red-Blue color scheme is used, with red representing the highest values and blue the lowest. White represents the marker average.
    • Note - Relative will give odd-looking results if only a small number of arrays are loaded.

GenePix Value Computation

Four methods for calculating a composite expression measurement for each marker in a GenePix file are offered. They combine the foreground and background and mean or median measurements from each channel in different ways.

  1. ( F635 Mean - B635 Mean) / (F532 Mean - B532 Mean).
  2. ( F635 Median - B635 Median) / (F532 Median - B532 Median).
  3. ( F532 Mean - B532 Mean) / (F635 Mean - B635 Mean).
  4. ( F532 Median - B532 Median) / (F635 Median - B635 Median).

See the entry GenePix Format in the GenePix tutorial chapter for further details of processing GenePix data on input.

Remote Components URL

geWorkbench can download certain components from a network repository. This specifies the location of the network component repository.

Marker Load Options

When a microarray dataset is loaded, along with its annotation file, it is possible to re-sort the markers at the global level - the new order will then be seen in all geWorkbench components. There are three options:

  • Load markers in the original order
  • Load markers ordered by gene name
  • Load markers ordered by probeset id

If no annotation file is loaded, sorting by probeset id can still be done.

If the Marker Load option is changed, it will take effect the next time a dataset is loaded.

In addition to this global sorting, markers can also be sorted by the same three methods in the Markers component (right-click on the marker list).

Remote Workspaces URL

Development versions of geWorkbench have a feature which allows a Workspace to be saved to a remote server for later retrieval or sharing.

Soft Limit on Cytoscape Networks Objects

There is a limit to the size of networks that Cytoscape can be used to visualize in geWorkbench. This limit will depend on the amount of memory allocated to geWorkbench. Viewing too large a network can cause geWorkbench to run out of memory and stop responding.

As of release 2.2.1, if the user attempts to view a large network in Cytocape, he or she will be offered the option to instead view the network using a built-in text viewer.

This "Soft Limit" preference setting controls the size of a network, calculated at the sum of the node (genes) and edges (interactions), that will trigger display of the dialog allowing the user to choose the text viewer. The user can experiment with attempting to view larger networks.

See the Cytoscape Network Viewer chapter for further details.

R Location (Rscript.exe and its folder)

Several geWorkbench components can make calls to a locally installed version of R. This field must contain the complete path to the R executable Rscript.exe.

Windows examples

If R is installed to the system directory, the complete entry may appear as shown here (C:\Program Files\R\R-3.0.2\bin\Rscript.exe:

RScript location system.png


If R is installed in the user's home directory, the complete entry may appear as follows (C:\Users\username\R\R-3.0.2\bin\Rscript.exe).


RScript location local.png


Mac OSX Example

Macintosh OSX users should install R in the usual default directory "Application". The path to the Rscript location becomes, e.g. for version 3.0: /Library/Frameworks/R.framework/Versions/3.0/Resources/Rscript.

Mac OSX Rscript location.png

The path to the R package directory can be left empty, however the packages will be installed in e.g. /Library/Frameworks/R.framework/Versions/3.0/Resources/library.

R package directory

Intended to hold the path to an alternate R package directory, if used.

  • Not working on Windows, instead use the Windows environment variable R_LIBS_USER.
  • This page was last modified on 22 April 2014, at 19:48.
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