Microarray Dataset Viewers
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In the this tutorial, you will:
- Get acquainted with the various geWorkbench visualization tools
- View a dataset in geWorkbench
- Modify the visualization preference settings
Visualization Tools
Visualization tools provide a view of the chip(s) under investigation and can be used for ascertaining the quality of the data. Activating gene and phenotype sets (Tutorials:Tutorial - Data Subsets) can be used to restrict the number of markers/arrays displayed. The images created can be saved and exported. A detailed description on how to manipulate visualization componenets is described in the online help.
| Microarray View: Used to inspect each separate microarray using the Array scroll bar. | 
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| Tabular Microarray Panel: Presents the numerical values of the expression measurements in a table format. One row is created per individual marker/probe and one column per microarray. | 
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| Color Mosaic: Heat maps for microarray expression data, organized by phenotypic or gene groupings. | 
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| Expression Profiles: This is a line graph of genes expression profiles across several arrays/ hybridizations.Each marker is a separate color line. | 
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| Scatter Plot: A pairwise (array vs. array and marker vs. marker) comparison and plotting of expression values. | 
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Preparation
Load a microarray dataset. This could be the dataset created in Tutorials: Projects and Data Files, or the file webmatrix.exp that is included with the geWorkbench download.
View a dataset
- Select the Microarray Viewer visualization component in the View Area at the top-right section of the interface.
- The All Markers and All Arrays checkboxes can be used to override the effects of activated sets, should they be in use. When no sets are activated, all markers or arrays will be used.
Preferences
The Preferences selection in the Tools menu allows users to specify how certain aspects of the system will behave. Once your preferences are set, these preferences are persistent between application sessions.
Modifying Settings
1. From the main menu, click on Tools>Preferences.
2. In the Preferences pop-up window, you can define settings for:
- Text Editor: The editor selected will be used to open and inspect data sets loaded in a project. Notepad is the default setting.
- Visualization: The color scheme to be applied to color mosaic images.
- Absolute: (default) Let M = max{|min|, |max|} over all expression measurements, across all arrays. If expression value x > 0, assign it the red spectrum x / M * 256. If expression value x is negative, assign it to the green spectrum -x / M * 256.
- Relative: This is similar to the setting for Absolute, but each marker is mean-variance normalized first.
- Genepix Value Computation: You can specify how compute the value displayed for Genepix array. The default setting is Option (Mean F635 - Mean B635) / (Mean F532 - Mean B532).
Select Relative for the visualization preference. Note that this choice will not take effect until the next time you load a data set.
3. Click on OK.
